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. 2017 Dec 27;12(12):e0189772. doi: 10.1371/journal.pone.0189772

Fig 5. CLAMP does not regulate NELF-B protein abundance.

Fig 5

(A) Transcript abundance for clamp and nelf-b was measured to determine the difference in transcript abundance between control (gfp, blue) and clamp (green) RNAi treatment by qPCR. The average fold change (ΔCt compared to gapdh) from four biological replicates is shown for both clamp and nelf-b transcripts. As expected, abundance of clamp is reduced after clamp RNAi, while nelf-b transcript abundance is not affected compared to the control RNAi. The error bars represent +/- 1 S.D., with p-values indicated. (B) Protein accumulation was measured by western blot for both CLAMP and NELF-B after of clamp and gfp control RNAi. RNAi targeting clamp reduces the amount of CLAMP protein but has no effect on NELF-B protein levels. Actin is used as a loading control.