Table 1. Physico-chemical parameters of various conjugates of PfCSP, Pfs25 and Pfs230D1 with EPA, TT or CRM197.
| Conjugate | Lot # | Conjugation Chemistry | Mol. Wt. distribution (kDa)a | Average. Mol. Wt. (kDa)a | Conjugate Composition Antigen/carrierb |
Antigen/carrier Mol. Wt. (kDa) |
|---|---|---|---|---|---|---|
| PfCSP-EPA | MV-8058 | TE | 259–4,238 | 1,075 | 1.72 | 32,578/66,983 |
| PfCSP-EPA | MV-8073 | Am | 223–3,390 | 1,140 | 2.1 | 32,578/66,983 |
| PfCSP-EPA | MV-8076 | GA | 291–1,840 | 797 | 1.6 | 32,578/66,983 |
| PfCSP-EPA | MV-8063 | ADH | 220–1,590 | 654 | 1.45 | 32,578/66,983 |
| Pfs25-EPA | MV- 8079 | TE | 184–1914 | 743 | 4.13 | 18,735/66,983 |
| Pfs25-EPA | MV-8080 | Am | 209–1878 | 1510 | 2.37 | 18,735/66,983 |
| Pfs230D1-EPA | MV- 8090 | TE | 170–1817 | 648 | 2.53 | 21,854/66,983 |
| Pfs230D1-EPA | MV-8093 | Am | 188–1767 | 690 | 2.08 | 21,854/66,983 |
| Pfs25-TT | MV-8081 | TE | 510–6,844 | 2,473 | 9.15 | 18,735/150,551 |
| Pfs25-CRM197 | MV-8085 | TE | 283–2,204 | 904 | 3.76 | 18,735/58,413 |
a: data from SEC-MALS analysis.
b: data obtained by Amino Acid Analysis of the conjugates and monomers.
TE: thioether chemistry; Am: Amide chemistry; GA: Glutaraldehyde chemistry; ADH: Adipic acid dihydrazide chemistry.