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. 2017 Dec 27;12(12):e0190241. doi: 10.1371/journal.pone.0190241

Fig 6. Maximal HIF-2 signaling during stress requires nuclear Acss2.

Fig 6

Semi-quantitative RTPCR measurement of HIF-1 selective (PGK1), HIF-2 preferential (MMP9, PAI1), or HIF-1/HIF-2 co-regulated (VEGFa, GLUT1) target genes in stably transformed control or Acss2 shRNA knockdown HT1080 cells, or stably transformed knockdown/rescue HT1080 cells expressing ectopic wild-type (WT) or cytosol-restricted mutant (CYT) Acss2 without or with an SV40 nuclear localization signal fused to the amino terminus after (A) early (4 hr) hypoxia or (B) late (24 hr) low glucose treatment. Comparison of samples within a given condition was performed by one-way ANOVA followed by Dunnett’s multiple comparisons test using control shRNA knockdown/control rescue as reference with decreased samples noted (*, P<0.05). Values indicated are means with SD.