Fig 10. Nuclear Acss2 regulates in vitro tumor cell properties.

(A) Cell proliferation of stably transformed knockdown/rescue HT1080 cells expressing wild-type (WT) Acss2, cytosol-restricted mutant (CYT) Acss2, or the same two proteins with the SV40 large T antigen nuclear localization signal fused to the amino terminus (SV40-WT, SV40-CYT). Cells were exposed to control, hypoxic, or low glucose conditions, or were supplemented with acetate (5 mM) (n = 8/treatment/day). (B) Colony formation, (C) Cell migration, or (D) Cell invasion after ten days of the same cells assessed under identical conditions (n = 3/treatment). Comparison of samples within a given condition on the day of interest was performed by two-way (cell proliferation) or one-way (colony formation, cell migration, cell invasion) ANOVA followed by Dunnett’s multiple comparisons test using control shRNA knockdown/control rescue as reference with decreased samples noted (*, P<0.05). For proliferation data, the indicated samples (*) differ from control cells for at least five of the last six days in this seven-day protocol. Other samples have no or one difference during this same period. Values indicated are means with SD.