Fig 12. Nuclear Acss2 regulates tumor cell growth and metastasis.

(A) Primary tumor weight as well as (B) luciferase activity and (C) lung metastatic burden assessed by luciferase activity in nude mice carrying flank tumors derived from luciferase-expressing stably transformed control or Acss2 knockdown HT1080 cells, or stably transformed knockdown/rescue HT1080 cells expressing wild-type (WT) Acss2, cytosol-restricted mutant (CYT) Acss2, or CYT Acss2 with the SV40 large T antigen nuclear localization signal fused to the amino terminus (SV40-CYT). Luciferase activity was assessed by triplicate measurements of tissue extracts. Mice in each group were treated with control or oral acetate (triacetin) delivered by oral gavage. Comparison within each shRNA knockdown/control rescue pair treated with vehicle or acetate (triacetin) was performed by one-tailed unpaired t test with Welch’s correction for groups of equal sample size or by one-tailed Mann-Whitney test for groups of unequal sample size with reductions indicated (*, P<0.05). Values indicated are means with SD (weights) or SEM (luciferase measurements).