Figure 6.

Insets of the spectra of Figure 5 with modification states of previous residue annotated, comparing the chemical shifts of C-terminal tails of tubulin with GST-fusion peptides. The peptides overlaid closely with the peaks corresponding to the unmodified form of the C-terminal tail residue. We observed significant chemical shift changes due to the change in the local chemical environment of the residue due to the presence of glycine modifications. Modifications are indicated by stars as in Figure 4.