Table 1.
Mutagen | Condition | Survival rate (%) | No. of colonies screened | No. of mutantsa (occurrence %) | Strains used in this study |
---|---|---|---|---|---|
NTG | 1 mg mL−1, 4 min | —b | 1248 | 5 (0.4) | |
1 mg mL−1, 7 min | — | 846 | 7 (0.8) | ||
1 mg mL−1, 10 min | 11 | 480 | 9 (1.9) | N9D9, N10E6 | |
2 mg mL−1, 10 min | 15 | 384 | 0 (0) | ||
1 mg mL−1, 20 min | 0.8 | 576 | 9 (1.6) | N3H4, N4H4, N6F4, N7C9 | |
Total | 3534 | 30 (0.8) | |||
GR | 772 Gy | 39 | 2168 | 6 (0.3) | G1G7, G17D5, G21E10 |
1158 Gy | 5 | 1080 | 13 (1.2) | G23C4, G23D3, G26C1 | |
Total | 3248 | 19 (0.6) |
Mutants that showed a clear phenotype in the second screening.
Not determined.