. 2017 Aug 1;7(9):6188–6197. doi: 10.1021/acscatal.7b01575

Table 3. Selected GOx Variants: Wild Type (WT), Parent (P), and Two Well-Performing Mutants^a.

GOx	K_M (mM)^b	k_cat (s^–1)^b	k_cat/K_M (mM^–1 s^–1)^b	t_1/2 (min)^b	⟨V_as⟩ (Å³)
WT	28.26 ± 1.15	189.38 ± 8.94	6.7	10.50 ± 0.71	261.9 ± 103.6
P	14.98 ± 0.51	291.82 ± 10.10	19.5	9.00 ± 0.70	238.3 ± 77.8
A2	18.54 ± 0.57	498.34 ± 15.12	26.9	11.74 ± 0.30	188.7 ± 65.2
F9	19.76 ± 0.54	345.16 ± 14.79	17.5	15.75 ± 0.71	239.3 ± 73.0

The enzyme kinetics was measured at pH 5.5 and the thermal stability was estimated on the basis of the half-life (t_1/2) at 60 °C.¹⁵ The average active site volume, ⟨V_as⟩, significantly decreases with increasing efficiency.

Data reproduced from Ostafe et al.¹⁵

Table 3. Selected GOx Variants: Wild Type (WT), Parent (P), and Two Well-Performing Mutantsa.

Table 3. Selected GOx Variants: Wild Type (WT), Parent (P), and Two Well-Performing Mutants^a.