Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers |
|---|---|---|---|
| strain, strain background (Shigella flexneri serotype 5a) |
M90T strain | PMID: 6279518 | Taxonomy ID: 1086030 |
| strain, strain background (Shigella flexneri serotype 5a |
mxiD | PMID: 8437520 | N/A |
| strain, strain background (Shigella flexneri serotype 5a) |
mxiE | PMID: 12142411 | N/A |
| strain, strain background (Shigella flexneri serotype 5a) |
ospG | PMID: 16162672 | N/A |
| strain, strain background (Shigella flexneri serotype 5a) |
ipaB | PMID: 1582426 | N/A |
| strain, strain background (Shigella flexneri serotype 5a) |
ipaC | PMID: 19165331 | N/A |
| strain, strain background (Shigella flexneri serotype 5a) |
ipaC/pC57 | PMID: 19165331 | N/A |
| strain, strain background (Shigella flexneri serotype 5a) |
ipaC/pC351 | PMID: 19165331 | N/A |
| strain, strain background (Shigella flexneri serotype 5a) |
virA | PMID: 22423964 | N/A |
| strain, strain background (Shigella flexneri serotype 5a) |
ipgD | PMID: 8478058 | N/A |
| cell line (Hela) | CCL-2 | ATCC | ATCC CCL2/CVCL_0030 |
| cell line (HT1080) | CCL-121 | ATCC | ATCC CCL-121/CVCL_0317 |
| cell line (HT1080) | GFP-PML-IV | PMID: 23530056 | N/A |
| cell line (Hela) | TAP-SUMO1 | PMID: 25097252 | N/A |
| cell line (Hela) | TAP-SUMO2 | PMID: 25097252 | N/A |
| cell line (HT1080) | UBC9-auxin degron | this paper | N/A |
| genetic reagent (siRNA) | control | Dharmacon | #D-001810–10 |
| genetic reagent (siRNA) | Capns1 | Dharmacon | #L-009979–00 |
| genetic reagent (siRNA) | Ubc9 | Dharmacon | #L-004910–00 |
| genetic reagent (siRNA) | SAE2 | Dharmacon | #L-005248–01 |
| genetic reagent (siRNA) | RhoGDIα | Dharmacon | #L-016253–00 |
| transfected construct (Plasmid) | pEGFP-RhoGDIα WT | PMID: 22393046 | N/A |
| transfected construct (Plasmid) | pEGFP-RhoGDIα K138R | PMID: 22393046 | N/A |
| antibody | anti-SAE1 | Abcam | #ab97523/AB_10681015 |
| antibody | anti-SAE2 | Abcam | #ab22104/AB_446785 |
| antibody | anti-SUMO1 | Abcam | Y299/AB_778173 |
| antibody | anti-SUMO2/3 | Abcam | 8A2/AB_1658424 |
| antibody | anti-UBC9 | Abcam | EP2938Y/AB_1267373 |
| antibody | anti-SUMO1 | DSHB Iowa | 21C7/AB_2198257 |
| antibody | anti-Calpastatin | Cell Signaling Technology | #4146/AB_2244162 |
| antibody | anti-SAE2 | Cell Signaling Technology | D15C11/AB_10889561 |
| antibody | anti-Cdc42 | Cell Signaling Technology | 11A11/AB_10695738 |
| antibody | anti-RhoA | Cell Signaling Technology | 67B9/AB_10693922 |
| antibody | anti-Tubulin | Cell Signaling Technology | DM1A/AB_1904178 |
| antibody | anti-RanGAP1 | Santa Cruz | C-5/AB_2176987 |
| antibody | anti-GFP | Santa Cruz | C-2 |
| antibody | anti-RhoGDIα | Merck Millipore | #06–730/AB_310229 |
| antibody | anti-Capns1 | Merck Millipore | MAB3083/AB_2070014 |
| antibody | anti-Rac1 | Merck Millipore | 23A8/AB_309712 |
| antibody | anti-LPS | PMID: 25097252 | N/A |
| antibody | anti-SP100 | PMID: 7559785 | N/A |
| chemical compound, drug | Phalloidin–Tetramethylrhodamine B isothiocyanate |
Sigma | P1951 / AB_2315148 |
| chemical compound, drug | Dapi | Sigma | D9542 |
| chemical compound, drug | Cytochalasin D | Sigma | C8273 |
| chemical compound, drug | MDL 28170 | Sigma | M6690 |
| chemical compound, drug | Ionomycin | Sigma | I3909 |
| chemical compound, drug | BAPTA-AM | Enzo life sciences | BML-CA411-0025 |
| chemical compound, drug | Indole-3-acetic acid | Sigma | I5148 |
| chemical compound, drug | N-Ethylmaleimide | Sigma | E3876 |
| peptide, recombinant protein | Recombinant SAE2 | Novus biologicals | NBP2-50574-20ug |
| peptide, recombinant protein | Recombinant Calpain-1 | Merck Millipore | 208712 |
| peptide, recombinant protein | GST-SENP2cat | this paper | NP_06760.2 |
| peptide, recombinant protein | SUMO1-AMC | Boston Biochem | UL-551 |
| peptide, recombinant protein | SUMO2-AMC | Boston Biochem | UL-758 |
| software, algorithm | Icy software | Institut Pasteur | PMID: 22743774 |