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Segmentation of zebrafish embryos with chorion using Hough-transformation circle detection algorithm. (a–c) Zebrafish embryos at different development stages: 3, 13, and 21.5 hpf, respectively. (d–f) Detailed structure of yolk, head, and tail of zebrafish embryos. H, head; T, tail; Y, yolk. The scale bars in (a)–(c) represent 1 mm, and those in (d)–(f) represent 0.3 mm.
