Figure 3.

The basic region uncharged residues enhance prothrombinase inhibition. (A) Prothrombinase activity was measured as in Figure 2A, in the presence of the indicated concentrations of TFPI-AAKA (filled square). The initial rate of thrombin generation is shown as a percentage of control (mean ± standard deviation; n ≥ 3). Lines represent best-fit inhibition curves. Inhibition of prothrombinase by TFPIα (open circle) and TFPIΔK2 (open square) is reproduced from Figure 2 for reference. (B-C) Thrombin generation was measured in TFPI-depleted plasma (B) or PRP (C) as in Figure 2, in the presence of the indicated concentrations of TFPI-AAKA. Shown are average thrombin generation curves from experiments performed in triplicate using TFPI-depleted plasma (B) or PRP from 4 donors (C).