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. Author manuscript; available in PMC: 2018 Oct 5.
Published in final edited form as: Mol Cell. 2017 Sep 21;68(1):76–88.e6. doi: 10.1016/j.molcel.2017.08.018

Figure 2. Chd1 monomer is sufficient for repositioning single nucleosomes back and forth.

Figure 2

(A) Single molecule traces of biotin-tethered nucleosomes, displaying initial repositioning (decrease in FRET) followed by repetitive increases in FRET, signifying sliding in the opposite direction.

(B) Schematic of surface-immobilized Chd1 and FRET-labeled nucleosome applied to single molecule platform.

(C) FRET histograms of non-biotinylated nucleosomes with surface-immobilized Chd1 before and after ATP addition.

(D) Single molecule traces of FRET labeled nucleosomes bound to surface-immobilized Chd1 before (top) and after (middle, bottom) ATP addition.