Table 1. Oligonucleotides for whole genome amplification and cloning.
Name | Sequence | Location (NC003748) | Predict length |
---|---|---|---|
EcPV1clone1F | CCTGGGGACGGTTCGCATTT | 6,550–6,569 | 1,788 |
EcPV1clone1R | GCATCTCTCACAGGCGTACC | 708–727 | |
EcPV1clone2F | GCCGTGTGTACCGTCTGCTG | 545–564 | 2,148 |
EcPV1clone2R | CCTGCTTTGCCTGTGATTGG | 2,673–2,692 | |
EcPV1clone3F | CGATGGGAGTCCTGTTTACC | 2,375–2,394 | 1,533 |
EcPV1clone3R | CCACCTTGTTTACAACGTCC | 3,888–3,907 | |
EcPV1clone4F | CCTTTACCCTAACCCCCTCCT | 3,680–3,700 | 1,721 |
EcPV1clone4R | GCACCCGCATGGTTTGGTCAC | 5,380–5,400 | |
EcPV1clone5F | GTTGATAGTCCAGACACCTCG | 5,110–5,130 | 1,660 |
EcPV1clone5R | CATCAGGCAAGAACGAGCAGG | 6,749–6,769 |