. 2017 Oct 20;79(12):1994–1997. doi: 10.1292/jvms.17-0358

Table 1. Antibodies used for immunohistochemistry.

Antibody	Dilution	Pretreatment^a	Antigen source	Manufacturer
Anti-vimentin (clone V9)	1:100	Heating^b	Monoclonal mouse	Dako, Carpinteria, CA, U.S.A.
Anti-cytokeratin (clones AE1/AE3)	1:500	Heating^b	Monoclonal mouse	Dako, Carpinteria, CA, U.S.A.
Anti-alpha smooth muscle actin (clone ASM-1)	1:700	Heating^b	Monoclonal mouse	Progen, Heidelberg, Germany

a) Pretreatment for antigen retrieval: b) heating: microwave heating with citrate buffer for 12 min at 600 w.