Fig. 3.

Root hair proliferation and expression of Pi transporter genes in osnla1 mutant and wild-type (WT). a Root hair proliferation of WT and osnla1 grown on Pi-sufficient (+P; left) and Pi-deficient (–P; right). Bars = 100 μm. b Root hair length in the maturation zone of roots. Data represent mean ± SD of eight replicates. Different letters represent significant differences according to Duncan’s multiple range test (P < 0.05). c Expression of Pi transporter genes in osnla1 mutant and WT. Ten-day-old plants grown in Pi-sufficient (300 μM Pi) nutrient solution were transferred to Pi-sufficient (+P) and -deficient (-P) conditions for 10 d. RNA was extracted from shoots (upper) and roots (lower) for qRT-PCR. Data represent mean ± SD of three replicates. Asterisks represent significant difference with the corresponding WT (*, P < 0.05; **, P < 0.01; ***, P < 0.001)