Table 2.

Phytochemical screening analysis

Test for phytochemicals	Amount of plant extract	Chemicals used	End point for confirmation of phytochemical
Carbohydrate	2 mL	Few drops of concentrated sulfuric acid and 1 mL of Molisch’s reagent	Reddish or purple color
Tannins	2 mL	4 mL of 5% ferric chloride	Greenish black or dark blue color
Saponins	2 mL	2 mL of distilled water and shake for 15 min	Layer of foam on surface
Flavonoids	2 mL	1 mL of 2 N sodium hydroxide	Yellow color
Alkaloids	2 mL	Few drops of Mayer’s reagent and 2 mL of concentrated HCl	White precipitate or green color
Anthraquinone	1 mL	Few drops of 10% ammonia solution	Pink color precipitates
Anthocyanosides	1 mL of filtrate	5 mL HCl	Pale pink color