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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: Exp Neurol. 2017 Oct 24;300:30–40. doi: 10.1016/j.expneurol.2017.10.022

Fig. 1.

Fig. 1

Experimental design and animal groups. Experiment 1: neurological tests were evaluated at 24h and 72h after surgery following which the animals were euthanized to collect brain samples for brain water content (BWC) measurement (n=6/group/time point). Experiment 2: animals were euthanized at 24h after surgery. Peripheral blood sample was collected during sacrifice for WBC count and LTB4 assay (n=8/group), and brain samples were harvested from the same animals for western blot (n=6/group) and immunohistochemistry (n=2/group). Experiment 3: neurological tests were evaluated at 24h following which animals were euthanized. Peripheral blood sample was collected during sacrifice for WBC count and LTB4 assay (n=8/group), after which brain samples were harvested from the same animals for western blot (n=6/group). Samples for SPC+SBI were shared with Experiment 2. Vehicle refers to ethanol. SBI, surgical brain injury; SPC, saline preconditioning, PPC, PLA2 preconditioning; BWC, brain water content, IHC, immunohistochemistry; WBC, white blood cells; LTB4, leukotriene B4.