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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: Toxicol Lett. 2017 Nov 24;283:100–105. doi: 10.1016/j.toxlet.2017.11.028

Figure 2.

Figure 2

N-acetylation of MOCA catalyzed by human recombinant NAT1 4 and NAT2 4. MOCA N-acetylation was concentration-dependent when catalyzed by both recombinant human NAT1 4 (circles) and NAT2 4 (squares). Each data point represents Mean ± SEM for three determinations. Michaelis Menten kinetics constants were calculated by non-linear regression and the best fit lines are illustrated. The apparent Km for recombinant NAT1 4 and NAT2 4 was 189 ± 25 and 48.4 ± 5.6 µM respectively in the presence of 1000 µM acetyl coenzyme A.