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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Transfusion. 2017 Aug 23;57(11):2595–2608. doi: 10.1111/trf.14288

Figure 2. IFNAR expression by conventional dendritic cells is dispensable for RBC alloimmunization.

Figure 2

Mixed chimeras were generated by reconstituting irradiated CD45.2+ WT recipients with a 4:1 mixture of Zbtb46-DTR (CD45.1+, CD45.2+) and either IFNAR1−/− (CD45.2+) or WT (CD45.1+) bone marrow. (A) Representative flow cytometric analysis of splenocytes derived from bone marrow donors in Zbtb46-DTR x IFNAR1−/− chimeras. (B) IFNAR1 expression of splenocytes gated in (A). (C) Representative flow cytometric analysis of spleen CD11c+ MHCII+ conventional dendritic cells in Zbtb46-DTR x IFNAR1−/− chimeras treated with PBS or diptheria toxin (DT) 8 weeks after bone marrow transfer. (D) Serum anti-KEL IgG produced by indicated chimeras treated with PBS or DT prior to transfusion with KEL RBCs. Z46-DTR = Zbtb46-DTR. Numbers on plots indicate percent of CD19− TCRβ − cells within the drawn gate. Representative of 3 independent experiments with 4–5 mice/group. n.s., not significant by Kruskal-Wallis test with a Dunn’s post-test. Data from repeated alloimmunization experiments are shown in Supplemental Figure 2A.