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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Transfusion. 2017 Aug 23;57(11):2595–2608. doi: 10.1111/trf.14288

Figure 3. T cell IFNAR expression is not required for RBC alloimmunization.

Figure 3

Mixed chimeras were generated by reconstituting irradiated IFNAR1−/− (CD45.2+) recipients with a 4:1 mixture of TCRα−/− (CD45.2+) and either IFNAR1−/− (CD45.2+) or WT (CD45.1+) bone marrow. (A–B) Representative reconstitution analysis of peripheral blood lymphocytes from (A) TCRα−/− x WT and (B) TCRα−/− x IFNAR1−/− chimeras. Numbers on plots indicate percent of live (left), CD45.2+ (middle), or CD45.1+ (right) cells within the drawn gate. (C) Relative expression of IFNAR1 on peripheral blood TCRβ+ T cells from IFNAR1−/− mice and the indicated chimeras. (D) Anti-KEL IgG in serum of indicated chimeras following transfusion with KEL RBCs. Representative of 3 independent experiments with 4–5 mice/group per experiment. n.s., not significant by Mann Whitney U test. Data from repeated alloimmunization experiments are shown in Supplemental Figure 2B.