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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Transfusion. 2017 Aug 23;57(11):2595–2608. doi: 10.1111/trf.14288

Figure 4. IFNAR expression by B cells is required for alloimmunization to KEL RBCs.

Figure 4

Mixed chimeras were generated by reconstituting irradiated WT (CD45.2+) recipients with a 2:1 mixture of muMT− (CD45.2+) and either IFNAR1−/− (CD45.2+) or WT (CD45.1+) bone marrow. Chimeras reconstituted with only IFNAR1−/− bone marrow served as negative controls for IFNAR1 expression and alloimmunization. (A–B) Representative reconstitution analysis of peripheral blood lymphocytes from (A) muMT− x WT and (B) muMT− x IFNAR1−/− chimeras. Numbers on plots indicate percent of live (left), CD45.2+ (middle), or CD45.1+ (right) cells within the drawn gate. (C) IFNAR1 expression on CD19+ B cells from indicated chimeras. (D) Serum anti-KEL IgG of indicated chimeras 28 days following transfusion with KEL RBCs. Representative of 3 independent experiments with 4–5 mice per group. **p<0.01 and n.s., not significant, by Mann Whitney U test. Data from repeated alloimmunization experiments are shown in Supplemental Figure 2C.