Figure 2.

Biochemical analyses of RNA–RNA interacting motifs that alternatively support the P5b–P6 interaction in the P5abc/ΔP5 bimolecular ribozyme. (A) Electrophoretic mobility shift assay (EMSA) of the P5abc/ΔP5 complexes in the presence of 10 mM Mg²⁺. L21-Tet Rz RNA (the parental unimolecular ribozyme from which the P5abc/ΔP5 complex was derived) and P5b-GGAA RNA were used as size markers for the complex and free-P5abc RNA, respectively. The asterisks indicate RNAs labeled with the BODIPY fluorophore. (B–D) Time courses of substrate-cleavage reactions catalyzed by the P5abc/ΔP5 bimolecular ribozymes in the presence of 3 mM Mg²⁺. Some error bars are small enough to be hidden by the symbols.