Figure 4.

Oligomeric RNA nanostructures whose assembly was mediated by the association between the P5abc module and the ΔP5 module. (A) Selective formation of closed tetramers by Lα RNA and Lβ RNA. (B) EMSA of Lα RNA + Lβ RNA in the presence of 20 mM Mg²⁺. The asterisks indicate monomer RNAs labeled with the BODIPY fluorophore. In lanes 1 and 5, the concentrations of Lα and Lβ were 0.5 μM. The concentrations of Lα + Lβ were 0.5 + 0.5 μM in lanes 2 and 6, 1.0 + 1.0 μM in lanes 3 and 7, and 2.0 + 2.0 μM in lanes 4 and 8. The concentrations of L2 + L3 were 0.5 + 0.5 μM in lanes 9 and 10. (C) One-dimensional open oligomers of F RNA and selective dimerization of its mutants. (D) EMSA of F RNA and its mutants in the presence of 50 mM Mg²⁺. The asterisks indicate monomer RNAs labeled with the BODIPY fluorophore. The concentrations of F RNA were 0.25 μM, 0.5 μM, 1.0 μM, and 2.0 μM in lanes 1, 2, 3, and 4, respectively. The concentrations of Fx RNA and Fy RNA were 0.5 μM in lanes 5 and 6. The concentrations of Fx RNA + Fy RNA were 0.5 + 0.5 μM in lanes 7 and 8.