Figure 1.

Overview of screen for mutants affecting curli production. (A) The Keio collection was screened on CR indicator plates after inoculation with a bolt replicator and incubation for two days at 26 °C. The collection was screened three times. (B) Associated phenotype scoring: 1 or white = csgA; 2 or light pink = hfq; 3 or pink = nagA; 4 or light red = greA; 5 or wild type (WT) = BW25113; and 6 or dark red = cpxR. (C) Distribution of Congo red (CR) phenotypes based on scoring from white to dark red. (D) Whole cell Western blots of strains probed with anti-CsgG and anti-CsgA antibodies.