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Lipopolysaccharide (LPS) mutants are defective for curli production. (A) LPS mutant strains and BW25113 (WT) grown on CR indicator plates at 26 °C for two days. (B) A schematic of LPS structure. Blue lines represent curli defective LPS mutants. Red lines represent LPS genes not in the Keio collection. (C) Whole cell Western blots of LPS mutants probed with anti-CsgG and anti-CsgA antibodies. All samples were grown on YESCA plates at 26 °C for two days and treated with formic acid.
