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MnP diminishes superoxide production in vitro. HepG2 cells were treated with 100 µM of PA, with or without 68 µM MnP, for 24 h. Cells were then loaded with DHE and read for fluorescence by flow cytometry. Unt: untreated, MnP: 68 µM MnP alone, PA: 100 µM PA alone, PA + MnP: 100 µM PA + 68 µM MnP. Data are displayed as mean fluorescence intensity (MFI) normalized to untreated controls ± SEM. Significance was determined by t-test of a combined n = 5 experiments (* indicates p < 0.05).
