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. 2017 Nov 1;6(4):85. doi: 10.3390/antiox6040085

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Redox modulation and NF-κB inhibition have anti-inflammation effects in the liver. After 12 weeks of HFD feeding, hepatic gene expression was measured by qRT-PCR for (A) MCP1; (B) TNFα; and (C) inducible nitric oxide synthase (iNOS), with or without MnP treatment. Data are displayed as mean ± SEM, n = 5 mice per group. After 12 weeks of HFD feeding, hepatic protein expression was measured by ELISA, for (D) MCP-1 and (E) TNF-α, with or without MnP treatment. Cytokine concentrations were normalized to protein concentration to yield pg of cytokine per mg of protein. Data are displayed as mean ± SD, n = 5 mice per group. Significance was calculated by t-tests. ** indicates p ≤ 0.01.