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. 2018 Jan;188(1):149–159. doi: 10.1016/j.ajpath.2017.09.010

Figure 2.

Figure 2

Architecture of the spleen in control and Notch2HCS mice. A: Representative paraffin sections derived from 6-month–old male mice stained with hematoxylin and eosin. Bottom panels show magnification of sections demonstrating enlargement of the white pulp (WP), the marginal zone (MZ), and the red pulp (RP) in Notch2HCS mutant and control mice. Large and small dots define the boundaries of the white and red pulp, respectively. B: Splenic architecture of control and Notch2HCS mice. Tile scans (top) of frozen sections stained with anti-CD169 Alexa Fluor 647 antibodies showing a characteristic perifollicular ring formed by CD169+ MZ macrophages. Bottom panels show images of representative follicles surrounded by macrophage and mature B-cell ring stained with anti-CD169 and anti-IgM-phycoerythrin antibodies. Vertical bars indicate the thickness of the MZ. The presence of nucleated cells was verified by DAPI staining (not shown). Scale bars: 1 mm (A and B, top panels); 200 μm (A, bottom panels); 250 μm (B, bottom panels).