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. 2018 Jan;188(1):149–159. doi: 10.1016/j.ajpath.2017.09.010

Figure 3.

Figure 3

Flow cytometry of spleen cells from control and Notch2HCS mice. A: Representative dot plot of flow cytometry of spleen cells from 2-month–old male and female Notch2HCS and sex-matched control littermates. Cells were stained with anti-CD21/35 and anti-CD23 antibodies. Circled areas define CD21/35highCD23− marginal zone B cells (MZBs) and CD21/35intCD23+ follicular B cells (FoBs) gated on B220+/CD19+IgM+ B-cell populations. B: Histogram of mean fluorescence intensity (MFI) of the CD21/35 cell population from 2-month–old male and female Notch2HCS and control mice. C: Bar graph of MFI of the CD21/35 cells for male and female 2-month–old control and Notch2HCS mice. D: Frequency of MZBs and FoBs from 1- and 12-month–old Notch2HCS mutant and wild-type control mice. Values are expressed as mean (95% CI) frequencies of MZBs and FoBs (A); data are presented as means and 95% CI (C and D). n = 4 biological replicates of control and experimental mice (C and D). ∗P < 0.05 versus control.