Figure 5.

Distribution of marginal zone B cells (MZBs) assessed by flow cytometry of spleen cells isolated from 2-month–old Notch2HCS mutant male mice and sex-matched littermate controls treated with anti–Notch2 negative regulatory region (NRR) antibody or anti-ragweed control antibody, both at 10 mg/kg for 4 weeks before sacrifice. A: Representative dot plots of spleen cells from 2-month–old control mice and Notch2HCS mice treated with anti-ragweed antibodies or anti-Notch2 NRR antibodies. Cells were stained with anti-CD21/35 and anti-CD23 antibodies. Circled areas define CD21/35^highCD23⁻ MZBs and CD21/35^intCD23⁺ follicular B cells gated on B220⁺/CD19⁺IgM⁺ B-cell populations. B: Frequencies of MZBs and follicular B cells (FoBs) in Notch2HCS mutant and wild-type littermates treated with anti-ragweed or anti-Notch2 NRR antibodies. Data are expressed as mean (95% CI) frequencies of MZBs and FoBs (A); data are expressed as means (95% CI) (B). n = 4 biological replicates. ^∗P < 0.05 Notch2HCS versus control; ^†P < 0.05 anti-ragweed antibody–treated versus anti-NRR antibody–wild-type mice; ^‡P < 0.05 anti-ragweed antibody–treated versus anti-Notch2 NRR antibody–Notch2HCS mice.