Table 3.
B-Cell Populations in the Bone Marrow and Spleen of 2-Month–Old Notch2HCS Mutant Mice and Sex-Matched Littermate Controls
| Gate used |
B220+IgM− |
B220+CD19+CD117− |
B220−CD19− |
|||
|---|---|---|---|---|---|---|
| Cell population | CD19−CD117− Pre-pro-B |
CD19+CD117+ Pro-B |
CD19+CD117− Pre-B |
IgM+ | IgMhigh | CD138+ plasma |
| Bone Marrow | ||||||
| Male | ||||||
| Control | 28.1 (22.3–33.9) | 1.2 (0.8–1.7) | 70.1 (64.3–75.9) | 43.7 (39.1–48.3) | 19.8 (13.9–25.7) | 1.1 (1.0–1.2) |
| Notch2HCS | 27.6 (23.9–31.2) | 1.9 (16–2.3) | 69.9 (65–73.3) | 39.7 (35.3–44.1) | 17.8 (15.6–19.9) | 1.3 (1.0–1.6) |
| Female | ||||||
| Control | 44.3 (37.2–51.4) | 7.4 (6.6–8.3) | 39.1 (32.3–45.9) | 55.6 (53.1–58.0) | 11.4 (10.2–12.6) | 2.4 (1.5–3.4) |
| Notch2HCS | 42.7 (37.3–48.1) | 7.0 (6.0–8.1) | 39.9 (32.6–47.1) | 55.1 (52.2–58.0) | 12.1 (19.3–14.9) | 2.0 (1.2–2.8) |
| Spleen | ||||||
| Male | NA | NA | NA | |||
| Control | 56.1 (46.9–65.4) | 20.5 (10.9–30.0) | 0.8 (0.3–1.3) | |||
| Notch2HCS | 51.0 (49.1–52.9) | 30.7 (28.6–32.9)∗ | 0.7 (0.6–0.9) | |||
| Female | ||||||
| Control | 64.6 (56.7–72.5) | 7.0 (2.4–11.6) | 0.4 (0.3–0.5) | |||
| Notch2HCS | 60.7 (56.2–65.3) | 23.2 (11.8–34.6)∗ | 0.4 (0.3–0.5) | |||
Data are expressed as mean (95% CI) percentages within the indicated gates of 4 biological replicates.
NA, not applicable.
∗
P < 0.05 Notch2HCS versus control.