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. 2018 Jan;188(1):111–124. doi: 10.1016/j.ajpath.2017.09.009

Figure 2.

Figure 2

Localization of insulin-like growth factor binding protein (IGFBP)-1 and casein kinase (CSNK)-2β using Image Pro-Premier 3D software version 9.2 (Media Cybernetics). A, C, and E: After performing dual immunofluorescence using anti-mouse IGFBP-1 (red) and anti-rabbit CSNK-2β (green) antibodies, we captured 63× magnified images on a confocal microscope. B, D, and F: To examine the localization of IGFBP-1 and CSNK-2β and their potential co-localization in HepG2 cells in more detail, iso-surface images were generated with Image Pro-Premier 3D software. Yellow rectangle outlines the cross section of the Z-stack–captured dual-immunofluorescence image. IGFBP-1 is present in the perinuclear region but not inside the nucleus; in addition, the abundance of IGFBP-1 appears to vary from cell to cell (B). CSNK-2β is clearly visualized throughout the cytosol and nucleus of HepG2 cells (D). Merged iso-surface image with the addition of a co-localization channel depicted as yellow, which is present in the perinuclear region of HepG2 cells (F). Scale bars: 10 μm.