Table 1.
Effect of oral treatment with Peganum harmala seeds extract on lung virus yield and mice survival in PR8 influenza virus-infected mice
| Group | Dead/total (M, %) | IP, % | MDD a , b | Viral RNA titer / β-actin gene expression b , c |
|---|---|---|---|---|
| PHS +virus | 3/7 (42.85) | 57.15 | 11.85±3.97 | 1.07×10-5± 7.87×10-6* |
| oseltamivir + virus | 2/7 (28.57) | 71.43 | 12.7±3.9* | 6.08×10-7± 5.03×10-7* |
| Virus control | 7/7 (100) | N/A | 8.57±2.07 | 8.43×10-5± 2.96×10-5 |
| PHS control | 0/7 (0) | - | >14 | N/A |
PHS: Peganum harmala seeds extract (200 mg/kg/day), oseltamivir (20 mg/kg/day), M: Mortality, IP: Index of protection,
a
MDD: Mean day to death of mice dying prior to day 14;
b
Data is represented as mean ± SD;
c
the levels of viral RNA were expressed as the ratio of viral RNA to β-actin mRNA (viral RNA/β-actin mRNA). Relative quantification of viral RNA to β-actin was determined using the 2−ΔCt = 2− (Ct, NS1− Ct, β-actin) equation.
*
p<0.05 versus virus control.