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. 2018 Jan;188(1):84–94. doi: 10.1016/j.ajpath.2017.09.015

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Supplemental Figure S1 — In embryonic day (E)14.5 control and Six2creFrs2α knock-out (KO) embryos, phosphorylated Creb (p-Creb) signal is present in Six2-positive nephron progenitors and Sox9-positive early nephron progenitor (NP) epithelial derivatives. A–F: Co-immunofluorescence for Six2 (red), p-Creb (red), or Sox9 (red) with E-cadherin (E-cadh) (green) on serial sections obtained from E14.5 control and Six2creFrs2αKO kidneys. A and B: In controls and Six2creFrs2αKO kidneys, Six2 signal is present in nephron progenitors (arrowheads) and E-cadherin stains the ureteric bud (arrows). C and D: In both control and Six2creFrs2αKO kidneys, p-Creb is present in Six2-positive nephron progenitors (arrowheads) in the E-cadherin–positive ureteric bud (arrows) and in the early epithelial nephron structures (concave arrowheads). E and F: Sox9 co-localizes with E-cadherin in the ureteric bud (arrowhead) in control and Six2creFrs2αKO kidneys, and also stains the early nephron epithelial structures (arrow). Scale bars: 50 μm.