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Embryonic day (E)13.5 kidney explants cultured in the presence of 8-Br-cAMP develop cysts. A and B: Representative stereomicroscopic images of kidney explants cultured in vehicle (A) or media containing 100 μmol/L 8-Br-cAMP (B) for 5 days. Cysts (concave arrowheads) can be first seen after 72 hours and continue to increase in size and number in the next 48 hours in the presence of 8-Br-cAMP. Scale bar = 500 μm.
