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. 2018 Jan;188(1):84–94. doi: 10.1016/j.ajpath.2017.09.015

Figure 1.

Figure 1

In control and Six2creFrs2α knock-out (KO) embryos, phosphorylated Creb (p-Creb) signal is present in nephron progenitors and early epithelial derivatives but absent in mature proximal tubules. A and B: Representative co-immunofluorescence images for p-Creb (red) and Ncam1 (green) in embryonic day (E)14.5 control (A) and Six2creFrs2αKO (B) kidneys shows p-Creb expression in Ncam1-positive nephron progenitors (concave arrowheads) and their immature epithelial derivative S-shaped bodies (arrows), as well as in Ncam1-negative ureteric bud epithelia (arrowheads). C and D: Co-immunofluorescence for p-Creb (red) and lotus tetragonolobus lectin (LTL) (green) in E18.5 control (C) and Six2creFrs2αKO (D) kidneys shows that p-Creb signal is absent in LTL-positive mature proximal tubules (concave arrowheads), but persistent in LTL-negative ureteric bud/collecting duct cells (UB) and stromal-derived interstitial cells (arrows). E and F: Co-immunofluorescence for p-Creb (red) and dolichos biflorus agglutinin (DBA) (green) in E18.5 control (E) and Six2creFrs2αKO (F) kidneys confirms a persistent p-Creb signal in the DBA-positive ureteric bud (arrowheads). Scale bars: 50 μm.