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. 2018 Jan;188(1):84–94. doi: 10.1016/j.ajpath.2017.09.015

Figure 5.

Figure 5

Ectopic phosphorylated Creb (p-Creb), Pax2, and Ncam1 expression in proximal tubule–derived cystic segments with decreased or absent lotus tetragonolobus lectin (LTL) staining in adult Ift88 knock-out (KO) kidneys 3 weeks after ischemia-reperfusion (IR). A, D, G, and J: Representative co-immunofluorescence images for p-Creb (red) and LTL (green) in control and Ift88KO, 3 weeks after IR injury or sham surgery. A: No p-Creb signal is present in control kidneys subjected to sham surgery (arrowhead), but it is present in non–LTL-positive cells that are likely interstitium (I) and collecting ducts (arrow). D: In control kidneys subjected to IR injury, p-Creb signal is observed in few LTL-positive proximal tubules (concave arrowheads) and in LTL-negative tubules (likely collecting ducts) (arrow), but absent in most LTL-positive proximal tubules (arrowheads). G: In Ift88KO kidneys subjected to sham surgery, no p-Creb signal is observed in LTL-positive proximal tubules (arrowhead), but is present in LTL-negative interstitium (I) and tubules (likely collecting ducts) (arrow). J: In Ift88KO/IR kidneys, p-Creb is absent in LTL-positive tubules and cystic cells (arrowheads), but is expressed ectopically in cystic segments with reduced or absent LTL staining (concave arrowheads). Arrow indicates p-Creb expression in interstitial cells. B, E, H, and K: Representative co-immunofluorescence images for Pax2 (red) and LTL (green). In the kidneys of control mice subjected to sham or IR injury surgery and Ift88KO kidneys subjected to sham surgery, Pax2 expression was seen only in LTL-negative tubules (likely collecting ducts) (arrows) and not in any LTL-positive tubules (arrowheads) (B, E, H). K: In Ift88KO/IR injury kidneys, Pax2 is absent in LTL-positive nondilated tubules and cystic cells (arrowheads), but is expressed ectopically in LTL-negative regions of cysts (concave arrowheads). Arrow indicates Pax2 expression in LTL-negative tubule (likely collecting duct). C, F, I, and L: Representative co-immunofluorescence images for Ncam1 (red) and LTL (green). In the kidneys of control mice subjected to sham or IR injury surgery and Ift88KO kidneys subjected to sham surgery, Ncam1 is not expressed in any LTL-positive tubules (arrowheads) (C, F, I). L: In Ift88KO/IR kidneys, ectopic Ncam1 signal is observed in LTL-negative regions of proximal tubule–derived cysts (concave arrowhead). Inset: Magnified view of the Ncam1-positive cell from boxed area. Ncam1 is not expressed in any LTL-positive tubules (arrowhead). Scale bars: 50 μm.