Skip to main content
. 2018 Jan;188(1):84–94. doi: 10.1016/j.ajpath.2017.09.015

Figure 7.

Figure 7

Exogenous 8-Br-cAMP in embryonic kidney explants results in cysts that are derived mostly from proximal tubules. Co-immunostaining with Aqp1 (red) and dolichos biflorus agglutinin (DBA) (green) or Aqp2 (red) and lotus tetragonolobus lectin (LTL) (green) in serial sections of vehicle or 8-Br-cAMP–treated embryonic day (E)13.5 kidney explants cultured for 5 days. A: Explants cultured in media and vehicle have normal Aqp1 (proximal tubule marker) and DBA (ureteric/collecting duct marker) staining with no cysts. B: Explants cultured in media and 8-Br-cAMP develop cysts that frequently co-label with Aqp1 (asterisks); although some cysts have partial loss of Aqp1 staining (concave arrowheads) and many Aqp1-expressing tubules are not dilated (arrowheads). None of the cysts in the 8-Br-cAMP–treated explants label for DBA (arrows). C: Explants cultured in media and vehicle have normal Aqp2 (collecting duct marker) and LTL (proximal tubule marker) staining with no cysts. D: Explants cultured in media and 8-Br-cAMP develop cysts that frequently co-label with LTL (asterisks), although some cysts have partial loss of LTL staining (concave arrowheads) and many LTL-expressing tubules are not dilated (arrowheads). None of the cysts in the 8-Br-cAMP–treated explants label for Aqp2 (arrows). Scale bars: 100 μm.