Lineage tracing shows that virtually all cysts induced by 8-Br-cAMP in embryonic kidney explants come from nephron progenitors. A–F: Fluorescent (A and B), brightfield (C and D), and merged images (E and F) of cryosections obtained from embryonic day (E)13.5 Six2creTg/+CAG-tdTomato+ kidney explants treated with vehicle or 8-Br-cAMP. A, C, and E: Vehicle-treated explants have no cysts. B, D, and F: Explants cultured in 8-Br-cAMP–containing media develop cysts, almost all of which express td-Tomato (red, asterisks). Scale bar = 100 μm.