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. 2018 Jan;188(1):84–94. doi: 10.1016/j.ajpath.2017.09.015

Figure 9.

Figure 9

Ectopic phosphorylated Creb (p-Creb) and Pax2 expression in proximal tubule–derived cysts induced by 8-Br-cAMP in wild-type kidney explants. Representative co-immunofluorescence images for p-Creb (red) and lotus tetragonolobus lectin (LTL) (green) (A and B) or Pax2 (red) and LTL (green) (C and D) on sections obtained from embryonic day (E)13.5 kidney explants cultured in media and vehicle or media and 8-Br-cAMP for 5 days. A: In vehicle-treated explants, p-Creb largely is absent in LTL-positive proximal tubule (arrowheads), although it is expressed in few LTL-positive tubules (concave arrowheads), in nephron progenitors (arrow), and a glomerulus (G). B and D: In 8-Br-cAMP–treated explants, p-Creb–positive (B) and Pax2-positive (D) cells are found in patterns similar to vehicle-only explants, but also are expressed ectopically in proximal tubule–derived cystic cells with decreased or absent LTL staining (concave arrowheads). C: Pax2 also largely is absent in LTL-positive tubules (arrowheads), but is found in a few LTL-positive cells (concave arrowhead) and in LTL-negative tubules (likely collecting ducts) (arrow). Scale bars: 50 μm.