Skip to main content
. 2013 Dec;183(6):1971–1980. doi: 10.1016/j.ajpath.2013.08.030

Figure 2.

Figure 2

Morphological and immunophenotypic features of NPM-ALK–transformed CD4+ T cells. A: H&E staining and immunohistochemical analysis for NPM-ALK, CD30, IRF4 (alias MUM1), CD3, and Ki-67 in NA1 cells. B: Multiparameter flow cytometry analysis of NA1 cells for expression of T-cell markers CD2 and CD3, and CD4 and CD25. C: Expression of IL-10 mRNA determined by RT-qPCR in NA1 and NA2 cells, with CD3 and CD28–stimulated, NPM-ALK–untransfected CD4+ T cells (Ctrl) serving as negative control. ALK+ ALCL cell lines SUDH-L1 and SUP-M2 served as positive controls. ∗P = 0.01 for the experimental versus control cells. D: Flow cytometry analysis of the NA1 and NA2 for CD30 expression. ALK+ ALCL–derived SUDHL-1 and mantle cell lymphoma–derived Jeko cell lines served as positive and negative control, respectively. E: Expression of the immunosuppressive PD-L1/CD274 protein by NA1, NA2, and control SUDHL-1 cells. Original magnification: ×200; ×400 (insets).