Figure 4.

Growth of the transformed CD4⁺ T cells in vivo. A: Ability of NPM-ALK–transfected CD4⁺ T lymphocytes to form tumors in immunodeficient mice. NA1 and control SUDHL-1 cells were transfected with a vector containing the luciferase gene, injected intraperitoneally at 3 × 10⁶ cells per mouse. The mice were examined for the presence of bioluminescence 5 weeks later. B: Flow cytometry detected expression of T-cell markers CD5 and CD3, and CD4 and CD3, by isolated tumor cells. C: Representative images of H&E staining and immunohistochemical staining for expression of NPM-ALK, CD30, CD2, CD3, and Ki-67 by the tumor tissues. Original magnification, ×400.