Table 1.
Label-Free Quantitative Tyrosine Phosphorylation Profile of a Subset of Average Normalized Abundances in the Tyrosine Phosphorylation Profile Determined from HCLEs Treated with Control (Media Change), ATP, or EGF
| Protein | Modifications | m/z | Running time (minutes) | Charge | ANOVA (P) (×10-2) | Fold | Average normalized abundances (×103) |
||
|---|---|---|---|---|---|---|---|---|---|
| Control | ATP | EGF | |||||||
| EGFR | p-Tyr-974 | 548.9004 | 30.4 | 3 | 0.0000104 | 46.7 | 13.8 | 69.5 | 325 |
| p-Tyr-1086 | 827.0708 | 24.7 | 3 | 0.000147 | 39.7 | 6.96 | 51.9 | 312 | |
| p-Tyr-1148 | 772.6703 | 33.9 | 3 | 0.000435 | 142.2 | 13.1 | 266 | 1870 | |
| p-Tyr-1173 | 759.0297 | 27.4 | 3 | 0.0000255 | 351.7 | 4.53 | 2.43 | 856 | |
| KPCD | p-Tyr-313 | 940.9012 | 29.7 | 2 | 0.00000962 | 13.67 | 6.65 | 91.2 | 23.3 |
| p-Ser-304 | 706.2921 | 29.2 | 3 | 0.000751 | 12.4 | 2.85 | 35.3 | 21.3 | |
| MAPK-13 | p-Tyr-182 | 765.3261 | 24.3 | 2 | 2 | 9.7 | 5.29 | 51.7 | 11.2 |
| p-Tyr-258 | 574.5929 | 25.7 | 3 | 0.0172 | 53.1 | 0.565 | 0.132 | 7.02 | |
| MAPK-12 | p-Tyr-185 | 816.8103 | 27.6 | 2 | 0.1 | 29.8 | 2.59 | 10.4 | 0.348 |
| MAPK-1 | p-Tyr-187 | 741.9948 | 29.7 | 3 | 1.7 | 23.7 | 130 | 1730 | 731 |
| p-Thr-190 | 1112.489 | 29.7 | 2 | 0.000184 | 88.7 | 8.74 | 70 | 0.789 | |
| p-Thr-185 | 768.6503 | 30.9 | 3 | 1 | 9.1 | 39.4 | 359 | 143 | |
| PLCG1 | p-Tyr-771 | 846.3689 | 29.1 | 2 | 0.000818 | 15.3 | 7.7 | 73.2 | 118 |
| p-Tyr-227 | 896.3942 | 36.5 | 2 | 1 | 8 | 1.66 | 3.65 | 13.3 | |
| RIN1 | p-Tyr-36 | 948.1138 | 27.9 | 3 | 0.2 | 11.9 | 26 | 310 | 133 |
| CDK2 | p-Tyr-15 | 541.2668 | 26.6 | 3 | 0.00000816 | 192.2 | 1.46 | 0.369 | 71 |
| CDC42-K | p-Tyr-827 | 739.3634 | 27.5 | 2 | 0.0676 | 29.1 | 1.6 | 11.3 | 0.389 |
| p-Tyr-859 | 605.3012 | 30.4 | 4 | 0.00207 | 49.5 | 2.3 | 14.6 | 114 | |
| p-Tyr-860 | 806.7332 | 30.5 | 3 | 0.0251 | 10.4 | 3.01 | 3.43 | 31.3 | |
| SHC1 | p-Tyr-427 | 1102.007 | 34.3 | 2 | 0.00303 | 196.7 | 2.45 | 1.62 | 319 |
| p-Tyr-315 | 457.5704 | 21.2 | 3 | 0.000967 | 401.5 | 0.546 | 0.122 | 48.8 | |
| SHB | p-Tyr-246 | 818.8424 | 25.7 | 2 | 0.000301 | 34.4 | 4.07 | 38.9 | 1.33 |
| p-Tyr-268 | 641.9358 | 24.1 | 3 | 0.7 | 8.2 | 4.38 | 3.27 | 26.8 | |
| p-Tyr-98 | 817.3299 | 28.5 | 2 | 0.0306 | 9.1 | 5.89 | 31.4 | 53.8 | |
| GAB1 | p-Tyr-659 | 636.7968 | 24.1 | 2 | 0.000000287 | 29.3 | 1.11 | 30.8 | 1.05 |
| p-Tyr-259 | 828.8849 | 31.5 | 2 | 0.01 | 14.8 | 4.86 | 72.2 | 19 | |
| p-Tyr-626 | 787.8527 | 33.8 | 2 | 0.00433 | 16.2 | 4.99 | 80.9 | 6.06 | |
| SRC8 | p-Tyr-446 | 750.98 | 27.9 | 3 | 0.008 | 11.5 | 1.84 | 20 | 21.1 |
| p-Tyr-421 | 530.9075 | 31.2 | 3 | 0.0411 | 27.3 | 8.55 | 23 | 6.36 | |
| PAXI | p-Tyr-88 | 676.6452 | 22.3 | 3 | 3.7 | 7.9 | 47.4 | 374 | 99 |
| p-Tyr-118 | 538.8987 | 24.8 | 3 | 4.5 | 12 | 357 | 4280 | 375 | |
| FAK | p-Tyr 576/577 | 783.3149 | 21.9 | 2 | 0.0575 | 64.6 | 1.75 | 0.193 | 12.5 |
| p-Tyr-861 | 880.432 | 28.2 | 4 | 0.000252 | 23.1 | 7.08 | 163 | 7.27 | |
| TYK2 | p-Tyr-259 | 627.8057 | 25.1 | 4 | 0.00481 | 35.8 | 8.11 | 79.3 | 290 |
| p-Tyr-292 | 800.3649 | 28.5 | 2 | 3.6 | 7.3 | 46.2 | 225 | 30.9 | |
| P85B | p-Tyr-464 | 794.8136 | 29.5 | 2 | 0.5 | 4.4 | 11.2 | 49.6 | 40.6 |
| p-Tyr-467 | 794.8136 | 29.5 | 2 | 0.5 | 4.4 | 11.2 | 49.6 | 40.6 | |
| FAK2 | p-Tyr-579/580 | 956.3759 | 24.4 | 2 | 0.00625 | 37.6 | 6.65 | 31.7 | 69.3 |
| PTK6 | p-Tyr-447 | 663.2683 | 29 | 2 | 0.2 | 30.8 | 18.9 | 147 | 583 |
| PTN11 | p-Tyr-62 | 908.3773 | 29.3 | 2 | 0.3 | 42.8 | 25.3 | 114 | 2.67 |
| p-Tyr-63 | 908.3773 | 29.3 | 2 | 0.3 | 42.8 | 25.3 | 114 | 2.67 | |
| PRP4B | p-Tyr-849 | 1259.0596 | 34.1 | 2 | 1.3 | 6.1 | 15.6 | 95.4 | 28 |
| p-Tyr-849 | 839.7086 | 34.2 | 3 | 1.4 | 10.3 | 105 | 1080 | 443 | |
| STAT3 | p-Tyr-705 | 646.2804 | 24.9 | 4 | 0.00455 | 15.4 | 15.3 | 122 | 18.7 |
| ITB4 | Y-1207 | 714.299 | 29 | 3 | 2.6 | 3.5 | 8.89 | 25.5 | 31.4 |
| CBL | p-Tyr-674 | 787.4292 | 33.3 | 3 | 0.0231 | 56 | 5.43 | 38.4 | 304 |
Statistical significance is calculated within the triplicate runs for each condition. Data were filtered based on P values (P < 0.05). Fold change significance was calculated using ANOVA.
ANOVA, analysis of variance.