Skip to main content
. 2013 Dec;183(6):1871–1884. doi: 10.1016/j.ajpath.2013.09.001

Figure 2.

Figure 2

Effect of ERK1/2 inhibition by the pharmacological inhibitor PD 98059 (100 μmol/L) on Caco-2 ERK1/2 activity, TER, and paracellular permeability. A and B: TNF-α (10 ng/mL) causes a time-dependent decrease in Caco-2 TER (A) and a time-dependent increase in mucosal-to-serosal flux of the paracellular marker inulin (B). C: Pretreatment with 100 μmol/L PD 98059 for 1 hour before TNF-α treatment inhibits the TNF-α–induced increase in ERK1/2 in vitro kinase activity. D: PD 98059 prevents the TNF-α–induced decrease in Caco-2 TER. E: Pretreatment with PD 98059 prevents the TNF-α–induced increase in mucosal-to-serosal inulin flux. Data are expressed as means ± SEM. n = 4 (C); n = 6 (A, B, D, and E). ∗P < 0.001 versus control. †P < 0.001 versus TNF-α treatment.