Skip to main content
. 2013 Dec;183(6):1871–1884. doi: 10.1016/j.ajpath.2013.09.001

Figure 4.

Figure 4

Effect of ERK1/2 inhibition on the TNF-α–induced increase in Caco-2 MLCK gene and protein expression. A: TNF-α causes a time-dependent increase in MLCK mRNA levels. The average copy number of MLCK mRNA in controls was 4.63 × 1011. B: TNF-α causes a time-dependent increase in MLCK protein expression. C: Pretreatment with the ERK1/2 inhibitor PD 98059 1 hour before TNF-α treatment prevents the TNF-α–induced increase in MLCK mRNA levels. D: The ERK1/2 inhibitor PD 98059 prevents the TNF-α–induced up-regulation of MLCK protein expression. E: siRNA-induced knockdown of ERK1/2 prevents the TNF-α–induced increase in MLCK mRNA. F: ERK1/2 siRNA transfection prevents the TNF-α–induced increase in MLCK protein expression. Data are expressed as means ± SEM. n = 8. Experimental period, 6 hours (C and E). ∗P < 0.001 versus control. †P < 0.001 versus TNF-α treatment.