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. 2013 Dec;183(6):1871–1884. doi: 10.1016/j.ajpath.2013.09.001

Figure 5.

Figure 5

Effect of TNF-α on Elk-1 activation. A: TNF-α treatment results in a time-dependent increase in Elk-1 phosphorylation. B: TNF-α causes a time-dependent increase in Elk-1 activity as determined by ELISA-based binding assay. C: Pretreatment with the ERK1/2 inhibitor PD 98059 for 1 hour before TNF-α treatment prevents TNF-α–induced Elk-1 phosphorylation. D: Pretreatment with PD 98059 for 1 hour before TNF-α treatment prevents TNF-α–induced binding of Elk-1 to its binding site on DNA probe as determined by DNA ELISA binding assay. Data are expressed as means ± SEM. n = 4. ∗P < 0.001 versus control. †P < 0.001 versus TNF-α treatment.