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. 2013 Dec;183(6):1871–1884. doi: 10.1016/j.ajpath.2013.09.001

Figure 6.

Figure 6

A: Elk-1 siRNA transfection results in a near-complete depletion in Elk-1 protein expression in filter-grown Caco-2 monolayers. B: Elk-1 siRNA transfection significantly prevents the TNF-α–induced increase in Caco-2 MLCK promoter activity. C: Elk-1 silencing by siRNA prevents the TNF-α–induced increase in Caco-2 MLCK mRNA levels. D: Elk-1 silencing by siRNA transfection abolishes TNF-α up-regulation of Caco-2 MLCK protein expression. E: Elk-1 siRNA transfection prevents the TNF-α–induced decrease in Caco-2 TER. F: Elk-1 silencing inhibits the TNF-α–induced increase in mucosal-to-serosal inulin flux. Data are expressed as means ± SEM. n = 4 (E); n = 5 (B and F); n = 8 (C). ∗P < 0.001, ∗∗P < 0.0001 versus control. †P < 0.001, ††P < 0.0001 versus TNF-α treatment.