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. 2017 Dec 1;6(1):182–191. doi: 10.1089/biores.2017.0023

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© Kathryn Taggart et al. 2017; Published by Mary Ann Liebert, Inc.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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FIG. 2. — AAV-mediated Gene Delivery. (A) A diagram of the vector constructs used in this study—control vector GFP driven by a CBA promoter (top—AAV-GFP) and therapeutic vector canine PDK4 driven by a CBA promoter (bottom—AAV-PDK4). Both promoter-transgene constructs are situated between AAV ITR to enable viral capsid packaging. (B) Timeline protocol of AAV treatment and fibroblast starvation. (C) Graph showing high level expression of PDK4 RNA transcripts in AAV-PDK4-treated PDK4^wt/del and PDK4^del/del fibroblasts compared to controls (*p < 0.05 and **p < 0.01 respectively). (D) Graph showing increased PDK4^del/del fibroblast viability in starvation conditions following AAV-PDK4 treatment (*p < 0.05). AAV, adeno-associated virus; CBA, chick beta actin; GFP, green fluorescent protein; ITR, inverted terminal repeats.