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. 2017 Dec 28;10:62. doi: 10.1186/s13041-017-0342-7

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — gef26 interacts genetically with rap1 at the NMJ. a Confocal images of anti-HRP-labeled NMJ 6/7 in wild-type and rap1 ^M/rap1 ^M third-instar larvae. Scale bar, 20 μm. b Quantification of total bouton number normalized to muscle surface area and satellite bouton number at NMJ 6/7 in wild-type, rap1 ^M/rap1 ^M, gef26 ⁶/+, rap1 ^M/+, gef26 ⁶/+; rap1 ^M/+, C155-GAL4/+, C155-GAL4/+; UAS-Myc-rap1 ^CA/+, gef26 ⁶/Df, and C155-GAL4/+; gef26 ⁶/Df; UAS-Myc-rap1 ^CA/+ backgrounds. The number of NMJs analyzed is indicated in each bar. Data are expressed as mean ± SEM. All comparisons are made with wild-type (*P < 0.001; **P < 0.01; ***P < 0.05)