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. 2017 Dec 28;17:124. doi: 10.1186/s12935-017-0495-6

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Matrine and cisplatin suppressed migration and invasion ability of EJ and T24 cells. Typical images of wound healing (a, e) and transwell assay (b, f) after 24 h treatment with 5 mM of matrine, 4 μM of cisplatin alone and 1 μM of cisplatin and 2 mM of matrine in combination. Histograms show the average migrated distance (c, g) and the amount of invasive cells (d, h), respectively. The protein expression levels of E-cadherin, β-catenin, Fibronectin, Vimentin and GAPDH of EJ and T24 cells after 24 h treatment with 4 μM of cisplatin, 5 mM of matrine and 1 μM of cisplatin and 2 mM of matrine in combination (i, j). Histograms present the relative grey value of the related proteins measured by ImageJ (k, l, m, n). All data are shown as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, or ****P < 0.0001 versus the control group (magnification, ×100; scale bars, 100 μm)